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Nitrogen is one of the most important limiting nutrients during tree growth. Oneyearold Acacia melanoxylon seedlings were selected in the experiment, and root characteristics under treatment of different N concentrations were studied. The results indicated that N supply could significantly increase the root morphology parameters such as root length, root mean diameter, root surface area and root volume (P<0.05). Effect of N total 400 mg for a plant was best to seedlings, by the end of the study, root length, root mean diameter, root surface area and root volume of seedlings were 343.35±20.60 cm, 0.41±0.02 cm, 26.30±1.32 cm2 and 0.35±0.03 cm3, respectively. Root growth treated by total 600 mg N for a seedling slowed down at the end of the experiment, perhaps high N concentration could harm seedling and restrain root growth.
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