2009年第2期
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一种改良的CTAB法提取马尾松基因组DNA

摘要:
以马尾松针叶为试验材料,利用改良的CTAB法抽提基因组DNA,其浓度和纯度符合PCR实验的要求,经凝胶电泳检测,DNA纯化效果好。
关键词:   改良CTAB法;马尾松;针叶;基因组DNA
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基金项目:福建省科技厅青年人才项目(2007F3017)
 
The genomic DNA extraction of Pinus massoniana using an improved CTAB method
Abstract:
The needles of Pinus massoniana were collected to extract the genomic DNA of P. massoniana by using the improved CTAB method. Its concentration and purity satisfied the request of PCR experiments. The gel electrophoresis demonstrated the good effect of DNA purification.
Key words:   improved CTAB method; Pinus massoniana; needle; genomic DNA